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KMID : 0363020080380040645
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Journal of Korean Academy of Periodontology
2008 Volume.38 No. 4 p.645 ~ p.655
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Effects of H2O2 and chlorhexidine on MMP-1, TIMP-1,2, Type 1 collagen, fibronectin and UNCL expressions in human periodontal Ligament fibroblasts
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Choi Seong-Mi
Jang Hyun-Seon
Kim Byung-Ock
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Abstract
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Purpose: To evaulate the effects of chlorhexidine and on matrix metalloproteinase-1 (MMP-1), tissue inhibitor of metalloproteinase(TIMP-1, TIMP-2), Type 1 collagen, fibronectin and UNCL expressions in human periodontal ligament fibroblasts (hPDLF).
Materials and Methods: , and CHX and , and and mixture of CHX and were applied to hPDLF for 1 min and 30 min. The mRNA expressions of MMP-1, TIMP-1 and 2, Type 1 collagen, fibronectin and UNCL in hPDLF were analysed by RT-PCR.
Results: The result were as follows: 1. The expression of UNCL mRNA was higher than that of other mRNAs. 2. CHX increased mRNA expressions of hPDLF as application time increased. 3. lower than increased expression of UNCL mRNA, and did not decrease mRNA expression of hPDLF. 4. hPDLF treatment with CHX (with or without ) resulted in no gene expression. 5. hPDLF treatment with CHX (with or without ) for 30 minutes resulted in no gene expression.
Conclusion: Because low concentration of CHX and increased UNCL mRNA expression of hPDLF, low concentraction of CHX and may have an antioxidative effect.
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KEYWORD
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chlorhexidine, H2O2, human periodontal Ligament fibroblasts
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